Journal: JCI Insight
Article Title: TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats
doi: 10.1172/jci.insight.137528
Figure Lengend Snippet: ( A ) The mRNA expression of α-SMA, collagen-1, and collagen-3 by real-time polymerase chain reaction (PCR). n = 4/group. ( B ) Proliferation of CFs by BrdU assay. Cells were treated with DMSO or GSK1016790A, or with GSK2193874 or GSK2193874 + multiple signaling pathway inhibitors. LY294002, an AKT inhibitor; S3I-201, a STAT3 specific inhibitor; SB 203580, P38 inhibitor; or SIS3, a SMAD3 inhibitor. n = 4/group, each in triplicate. Statistical analyses: 1-way ANOVA with Bonferroni’s post hoc test ( D ); * P < 0.05, ** P < 0.01, *** P < 0.001 versus DMSO; # P < 0.05, ## P < 0.01, ### P < 0.001 versus GSK1016790A. Data are expressed as the mean ± SEM.
Article Snippet: Atrial fibroblasts proliferation was measured using a FITC- bromodeoxyuridine (BrdU) cell proliferation Detection Kit (KeyGEN BioTECH) following the manufacturer’s instructions.
Techniques: Expressing, Real-time Polymerase Chain Reaction, BrdU Staining